Transferência do Gene de VEGF para a Doença Cardiovascular (Inglês)

Peter R. Vale M.D, FRACP . . Departments of Vascular Medicine, Cardiology and Cardiovascular Research, St Elizabeth's Medical Center, Tuft's University School of Medicine, Boston, MA, USA. . Abstract. . Evidence that VEGF stimulates angiogenesis in vivo had been developed in experiments performed on rat and rabbit cornea, the chorioallantoic membrane, and the rabbit bone graft model. It is now clear that bone-marrow derived endothelial progenitor cells contribute to such neovascular networks as well.. . Preclinical studies have established proof of principle for the concept that the angiogenic activity of VEGF is sufficiently potent to achieve therapeutic benefit. Doses of 500-1,000 µg of phVEGF165 produced statistically significant augmentation of angiographically visible collateral vessels, and histologically identifiable capillaries in rabbits with severe, unilateral hindlimb ischemia. Similar results were achieved in a separate series of experiments in which VEGF was administered by an intramuscular route daily for 10 days.. . Site-specific transfection of genes encoding for a secreted protein may overcome the handicap of inefficient transfection by a paracrine effect, even when the number of transduced cells remains low, by secreting adequate protein to achieve local levels with physiologically meaningful biological effects. In vitro and ...

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